Regina Westminster Pastoral Charge was formed as a new United Church charge in 1925, including Westminster United Church, itself an amalgamation of Westminster Presbyterian Church (at 3025 - 13th Avenue) and the nearby Fourteenth Avenue Methodist Church (in a temporary building on the southwest corner of 14th Avenue and Retallack Street). These congregations had originated from outreach efforts by the downtown Knox Presbyterian Church and Metropolitan Methodist Church and, in May 1925, they had combined their congregations, in the formerly Presbyterian building.

Regina Zion Pastoral Charge was formed as a new United Church charge in 1925, containing Zion United Church, in Regina. Around 1932, Zion was listed as part of Regina Westminster Pastoral Charge but the points later re-formed into separate charges. Regina Zion Pastoral Charge officially closed on June 30, 2015.

Saskatchewan Anti-Tuberculosis Commission, often mistakenly called the Royal Commission on Tuberculosis set out to "to enquire into the question of tuberculosis in Saskatchewan, and to recommend...measures to efficiently deal with the problem" (Report of the Saskatchewan. Anti-Tuberculosis Commission, A.B. Cook, 1922).

Regina Presbytery was one of the original 16 presbyteries established by the first General Council of the United Church of Canada (June 1925), to be part of the new Saskatchewan Conference. Initial boundaries were based on the previous Regina Presbytery, which had been part of the Presbyterian Synod of Saskatchewan. Prior to Union, in 1925, there had also been a Regina District, in the Saskatchewan Conference of the Methodist Church.

In 2000, Saskatchewan Conference went from 10 presbyteries to 7, with each adopting a new name. Most pastoral charges from Regina Presbytery became part of the new Wascana Presbytery.

Wascana Presbytery was one of the 7 presbyteries of Saskatchewan Conference created in 2000. It was initially comprised of pastoral charges from the former Regina Presbytery. In January 2019, as part of a larger restructuring of the United Church of Canada, Wascana Presbytery was combined with Saskatchewan Conference and the other remaining presbyteries to become Region 4.