The United Church of Canada was formed in 1925, from a union of the Congregational Union of Canada, the Methodist Church and 70% of the Presbyterian Church in Canada, along with a number of churches organized under the General Council of Local Union Churches (mostly in Western Canada). Saskatchewan Conference was one of eleven regional Conferences established by the United Church of Canada General Council, at their inaugural meeting, in June 1925. Initially, the Conference contained 16 presbyteries but this number varied, in the years that followed.

In January 2019, as part of a larger restructuring of the United Church of Canada, Saskatchewan Conference and its seven existing presbyteries were combined into a single entity, initially identified as Region 4 and later renamed Living Skies Regional Council.

Arcola-Estevan Presbytery was created from the amalgamation of Arcola Presbytery and Estevan Presbytery, in 1951. In 1973, Arcola-Estevan Presbytery was combined with part of Assiniboia Presbytery, becoming the new Souris Valley Presbytery.

Cypress Hills Presbytery was created, in 1959, from the merger of Swift Current Presbytery and the part of Assiniboia Presbytery west of Highway 19.

In 2000, Saskatchewan Conference went from 10 presbyteries to 7, with each adopting a new name. Most pastoral charges from Cypress Hills Presbytery became part of the new Chinook Presbytery.

Qu’Appelle Presbytery was one of the original 16 presbyteries established by the first General Council of the United Church of Canada (June 1925), to be part of the new Saskatchewan Conference. Initial boundaries were based on the previous Qu’Appelle Presbytery, which had been part of the Presbyterian Synod of Saskatchewan.

In 2000, Saskatchewan Conference went from 10 presbyteries to 7, with each adopting a new name. Most pastoral charges from Qu’Appelle Presbytery became part of the new Twin Valleys Presbytery.

Saskatoon Presbytery was one of the original 16 presbyteries established by the first General Council of the United Church of Canada (June 1925), to be part of the new Saskatchewan Conference. Initial boundaries were based on the previous Saskatoon Presbytery, which had been part of the Presbyterian Synod of Saskatchewan. Prior to Union, in 1925, there had also been a Saskatchewan District, in the Saskatchewan Conference of the Methodist Church.

In 2000, Saskatchewan Conference went from 10 presbyteries to 7, with each adopting a new name. Most pastoral charges from Saskatoon Presbytery became part of the new River Bend Presbytery.

The Provincial Church Union Committee was formed on July 26, 1923, by representatives of the Saskatchewan Methodist Conference and the Presbyterian Synod of Saskatchewan to take preliminary steps to effect Church Union in Saskatchewan. Representatives of the congregational Churches were also invited to be on the committee.

The Joint Committee officially convened in April 1904, in Toronto, bringing together appointed representatives from the Congregationalist, Methodist and Presbyterian Churches, to negotiate church union. Meetings continued through to 1908, when the terms written in the Basis of Union were agreed upon and sent to the negotiating churches, for discussion and approval. By 1912, both the Congregationalists and the Methodists had agreed to the terms. The decision was more contentious for the Presbyterian Church, though in 1916, their General Assembly decided to go ahead with the union.
Between 1916 and 1925, the Joint Committee worked to complete the union and defeat those opposing it, including the newly formed Presbyterian Church Association.