Assiniboia Presbytery was one of the original 16 presbyteries established by the first General Council of the United Church of Canada (June 1925), to be part of the new Saskatchewan Conference. Initial boundaries were based on the previous Assiniboia Presbytery, which had been part of the Presbyterian Synod of Saskatchewan.

The presbytery was divided and recreated several times, over the years. In 1926, it was split into Assiniboia Presbytery and the new Shaunavon Presbytery. In 1951, the two were reunited under the original name Assiniboia Presbytery. In 1959, the presbytery was divided along Highway 19: the western part was combined with Swift Current Presbytery, to become Cypress Hills Presbytery, and the eastern part was amalgamated with Weyburn Presbytery, under the name Assiniboia Presbytery.

In 1973, Assiniboia Presbytery was dissolved and its pastoral charges were divided between Moose Jaw Presbytery and Arcola-Estevan Presbytery (which became the new Souris Valley Presbytery).

Battleford Presbytery was one of the original 16 presbyteries established by the first General Council of the United Church of Canada (June 1925), to be part of the new Saskatchewan Conference. Initial boundaries were based on predecessor Battleford Presbytery, which had been part of the Presbyterian Synod of Saskatchewan.

In 2000, Saskatchewan Conference went from 10 presbyteries to 7, with each adopting a new name. Most pastoral charges from Battleford Presbytery became part of the new Prairie Pine Presbytery.

Cypress Hills Presbytery was created, in 1959, from the merger of Swift Current Presbytery and the part of Assiniboia Presbytery west of Highway 19.

In 2000, Saskatchewan Conference went from 10 presbyteries to 7, with each adopting a new name. Most pastoral charges from Cypress Hills Presbytery became part of the new Chinook Presbytery.

The Cooperating committee of Saskatchewan was formed when representatives of the Presbyterian and Methodist Churches met at Regina, May 2, 1911, in order to facilitate cooperative activity in various localities in Saskatchewan. The formation of this Committee paralleled a similar action taken at the national level by the Presbyterian, Methodist and Congregational Churches. After mid-1923, this committee was called the Provincial Committee on Co-operation.

The Joint Committee officially convened in April 1904, in Toronto, bringing together appointed representatives from the Congregationalist, Methodist and Presbyterian Churches, to negotiate church union. Meetings continued through to 1908, when the terms written in the Basis of Union were agreed upon and sent to the negotiating churches, for discussion and approval. By 1912, both the Congregationalists and the Methodists had agreed to the terms. The decision was more contentious for the Presbyterian Church, though in 1916, their General Assembly decided to go ahead with the union.
Between 1916 and 1925, the Joint Committee worked to complete the union and defeat those opposing it, including the newly formed Presbyterian Church Association.

The Provincial Church Union Committee was formed on July 26, 1923, by representatives of the Saskatchewan Methodist Conference and the Presbyterian Synod of Saskatchewan to take preliminary steps to effect Church Union in Saskatchewan. Representatives of the congregational Churches were also invited to be on the committee.

Elrose Presbytery was created in 1926, from the division of Kindersley Presbytery. Early pastoral charges in the presbytery included Eston, Dinsmore, Ferndale (student field), Richlea, Lucky Lake, Annerly (student field), Tuberose, North Landing, Demaine-Beechy, Elrose, Plato, Macrorie, Wiseton, Buffalo Basin (student field), Glidden, Wartime, Dunblane (including BIrsay), Dunblane (Finnish), Eatonia (English and German congregations), Rankin (student field), Mantario, Lacadena, Sanctuary.

In 1949, the Presbyteries of Elrose and Kindersley amalgamated, to become Rosetown Presbytery.

Arcola-Estevan Presbytery was created from the amalgamation of Arcola Presbytery and Estevan Presbytery, in 1951. In 1973, Arcola-Estevan Presbytery was combined with part of Assiniboia Presbytery, becoming the new Souris Valley Presbytery.

Estevan Presbytery was the new name given to Alameda Presbytery, by the first Saskatchewan Conference (October 1925). As Alameda Presbytery, it was one of the original 16 that were established by the first General Council of the United Church of Canada (June 1925), to be part of the new Saskatchewan Conference. Initial boundaries for both Alameda Presbytery and Weyburn Presbytery were to follow the recommendation of a joint meeting of Alameda and Weyburn Presbyteries, from the Presbyterian Synod of Saskatchewan, and the Weyburn District of the Methodist Saskatchewan Conference.

In 1951, Arcola and Estevan Presbyteries amalgamated, becoming the Arcola-Estevan Presbytery.